Water for Cell Culture | what type of water should be used for cell culture?

Water is extensively used in the key procedures of cell culture process. It is mainly used as a solvent in the preparation of buffers and media, other roles including the dissolution of additives and drugs, rinsing of bioreactors, plastic ware and glassware, etc… Contaminants in water may influence the growth, morphology as well as behavior of cultured cells, thereby affecting the outcome. It is necessary to choose the right water with quality up to standards to perform cell culture. Besides, monitoring and maintaining water quality throughout the process plays an important part in running predictable and repeatable experiments. Thus, how to select a reliable water purification system for this application is one of the key concerns of researchers.

Cell Culture water CLSI

Effects of common impurities in tap water on cell culture

Impurities from tap waterEffects on cell culture
(Inorganic) IonsToxic to different cells
Inhibits the reactions
Cause side reactions
BacteriaCause pH changes in media
Contaminate incubator or hood
EndotoxinsChange cell shapes
Cell adhesion or division
Change the growth and functions of cells
Affect cloning efficiency, and production of recombinant proteins
TOCAffect the development of cells
Cell Culture grade water CLSI

Referring to the guidelines provided by ISO3696, CLSI (Clinical and Laboratory Standards Institute), ASTM International (American Society for Testing and Materials), EMA (European Medicines Agency) and USP (US Pharmacopeia), fresh ultrapure water (Type I water) is strongly recommended for cell cultivation, and the highest specification is required to avoid contaminants introduction, as summarized in the table below.

Cell Culture Grade Water requirements

Cell Culture Grade Water Standard

Special care needs to be taken to make sure that the water used for cell culture is free from any inorganic ions (especially heavy metals), organic compounds, pyrogens and bacterial.

About RephiLe & Duoning:

RephiLe, Revolution in Filtration. RephiLe Bioscience designs and manufactures innovative and high-quality purification-based products for biotechnology and life science laboratories. Our offering includes laboratory ultrapure water systems, replacement consumables, and other filtration products. It is RephiLe’s commitment to helping our customers beyond the products which we manufacture.

Duoning is a one-stop solution provider in the field of the biopharmaceutical industry and laboratories. With an integrated product portfolio covering almost all major steps of biopharmaceutical manufacturing including plasmid construction, production of adeno-associated (AAV) vectors, and mRNA vaccines, Duoning aims to enhance customers’ productivity by offering fast and effective tools from bench through bioprocess production.