Which grade of water to use for Silver Staining
Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. Among the various protein detection methods, the technique of silver staining has gained wide popularity because of its higher sensitivity (in the low ng range) than that of Coomassie blue. Meanwhile, it can be achieved with simple and inexpensive laboratory reagents and no complicated and expensive hardware for the readout is required.
Silver staining now is commonly used in the field of environmental science, botany, breeding science, etc., for example, it aids the visualization of electrophoretically separated proteins in Histological characterization research. It can be also used for diagnostic microbiology, karyotype analysis, genomic and proteomic analysis and many other applications due to its high sensitivity and low cost.
During the procedure of silver staining, many factors may have influence on the final staining results. Has it ever occurred to you that the water you use for the overall experiment has played a very important role in deciding whether it is a successful staining or not?
For example – for optimal sliver staining results, the level of chloride ion (Cl–) in the water used must be kept as low as possible. If the amount of chloride ion present in the water or reagents reaches 2 ppm under normal conditions, precipitation is likely to take place and a big haze appears on the gel surface at the development stage. A terrible background results in a failure of your experiment.
One thing you must know- silver staining is very sensitive to trace impurities in water. It is critical that water with a resistivity greater than 15MΩ/cm must be used.
In brief, only EDI pure water or ultrapure water can be used in silver staining, while RO pure water cannot give you a satisfactory result!
EDI is a technology that combines the semi-permeable membrane with ion-exchange media to provide a higher efficiency demineralization process than that of RO.
RephiLe’s latest powerful and revolutionary Genie G water systems can produce EDI pure water and ultrapure water simultaneously
◆EDI pure water – The water quality fully satisfies the silver staining and can also be used in many other routine and non-critical applications.
◆Ultrapure water – Can be applied to the upstream and downstream of silver staining as well as other protein research experiments, such as protein SDS-PAGE electrophoresis, Western Blotting and so on.
Furthermore, a RephiBio final filter can be installed on the dispenser outlet which ensures ultrapure water free of particles, bacteria, pyrogen, RNAses or DNAses.
A Genie G ultrapure water system provides you with an integrated solution for lab water supply.
RephiLe water systems for better solutions!
Driven by innovation and quality, RephiLe is a dedicated provider of water purification systems and laboratory filtration products. At the same time, RephiLe produces comprehensive consumables that can be used in Millipore lab water systems with reliable performance. It is RephiLe’s commitment to becoming a partner of choice for customers in the area of life science and biotechnology. The company is striving to bring superior quality, high value and innovative purification tools to enable and accelerate the advancement of the life sciences and technologies. Products are being sold to over 90 countries worldwide.
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